Letty Hughes was born in Montana, but her family moved to interior Alaska when she was about six years old. She graduated from the University of Alaska Fairbanks in 2004 with an undergraduate degree in wildlife biology. She has worked for the Alaska Department of Fish and Game in Fairbanks, Bethel and Nome, Alaska, and since July 2018 has been the wildlife biologist for Bering Land Bridge National Preseve based in Nome. Her work has included being a field technician studying the stomach contents of seals to conducting animal population surveys of moose, caribou, bears, muskoxen, and wolves. She has also looked at vegetation and changes to the animals' habitats and is involved with the Bering Land Bridge and the Arctic Inventory and Monitoring Network.